Application of the SDS–PAGE method to 22 species of
fish revealed a marked variation in parvalbumin content among fish. Furthermore, the parvalbumin
content was found to be higher in dorsal white muscle than in ventral white muscle, in rostral part of
white muscle than in caudal part of white muscle and in white muscle than in dark muscle. IgE reactivity
of fish was roughly proportional to parvalbumin content. Interestingly, large-sized migratory fish, such as
salmon, swordfish and tuna, were commonly very low in both parvalbumin content and IgE reactivity.