The aim of this study was to compare the effect of two different types of oils in diet on the fatty acid
profile in the eggs of layers and to include a particular type of oil as a supplement of feeding mixtures for
layers in order to support the development of functional foodstuffs. Thirty layers fed a diet containing
soybean oil constituted the control group (soybean oil is the most frequently used oil added to feeding
mixtures). In the experimental group (thirty layers), soybean oil was replaced with linseed oil at the
same amount (3 kg of oil per 100 kg of feeding mixture). Feeding was provided ad libitum for all
days of the month. After one month, egg yolks were analysed and the fatty acid profile was compared.
Significant differences (P ≤ 0.05) were found in the concentration of myristic acid that belongs to the
group of saturated fatty acids. Eggs in the experimental group showed higher concentrations of myristic
acid compared to the control group (0.20 g/100 g of fat and 0.18 g/100 g of fat, respectively). Highly
significant differences (P ≤ 0.01) were found for heptadecanoic acid but the trend was opposite to that
of myristic acid; concentrations of heptadecanoic acid in the experimental group were lower than those
in the control group. Highly significant differences (P ≤ 0.01) were found for n-9 monounsaturated
fatty acids where egg yolks in eggs from layers fed linseed oil contained higher concentrations
of oleic acid, myristoleic acid, and palmitoleic acid. Lower concentrations of n-6 fatty acids
(P ≤ 0.01) were found after the addition of linseed oil in eggs. Linseed oil showed a positive effect
on n-3 fatty acids (α-linolenic acid), its concentration in the control and experimental group was 0.82
g/100 g of fat and 5.63 g/100 g of fat, respectively. The possibility of influencing the fatty acid profile
in eggs is very important for the development of functional foods.