For the cultures grown at 20 C, the bacterial count exceeded
107 CFU/mL under all pH conditions after culturing for 12 h, which
was also accompanied by an accumulation of histamine. After
culturing for 18 h, the bacterial count exceeded 108 CFU/mL under
all pH conditions, and the amount of histamine exceeded 500 ppm
(Fig. 3a and b).
Although the bacterial proliferation did not vary much with the
pH at each culturing temperature, the amount of histamine produced
every hour increased with the decreasing pH. The results of
the significance tests showed that for each temperature condition,
the amounts of histamine produced at the lowest pH (pH 5) and
highest pH (pH 7) at the time of the final collection (day 7 for the
5 C cultures, 72 h for the 10 C cultures, and 48 h for the 20 C
cultures) were significantly different (p < 0.01). Moreover, for the
20 C cultures, the amount of histamine produced at pH 7 was
significantly different from the amounts of histamine produced at
other pHs (p < 0.01 for pH 5, 5.5, 6 vs. pH 7, and p < 0.05 for pH 6.5
vs. pH 7).
For the cultures grown at 20 C, the bacterial count exceeded107 CFU/mL under all pH conditions after culturing for 12 h, whichwas also accompanied by an accumulation of histamine. Afterculturing for 18 h, the bacterial count exceeded 108 CFU/mL underall pH conditions, and the amount of histamine exceeded 500 ppm(Fig. 3a and b).Although the bacterial proliferation did not vary much with thepH at each culturing temperature, the amount of histamine producedevery hour increased with the decreasing pH. The results ofthe significance tests showed that for each temperature condition,the amounts of histamine produced at the lowest pH (pH 5) andhighest pH (pH 7) at the time of the final collection (day 7 for the5 C cultures, 72 h for the 10 C cultures, and 48 h for the 20 Ccultures) were significantly different (p < 0.01). Moreover, for the20 C cultures, the amount of histamine produced at pH 7 wassignificantly different from the amounts of histamine produced atother pHs (p < 0.01 for pH 5, 5.5, 6 vs. pH 7, and p < 0.05 for pH 6.5vs. pH 7).
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