Transesterification of 100 L jatropha oil was carried out on the pilot scale with the same chemical conditions used in the bench scale experiment. The reaction was carried out in an enameled reactor of 150 L capacity equipped with an anchor stirrer and provided with temperature, pressure and stirring speed controllers. Jatropha oil was loaded to the reactor and preheated. The solution of the methanol and sodium hydroxide was prepared and added to the reactor. The reactants