The mice (or rats) used should be the same
strain (syngenic) both for immunization to
produce the hybridoma clone and for subsequent
mAb production, so that the tissue
used is histocompatible. BALB/c mice are
often the animal of choice, as many of the
parental myeloma cells used in the fusion
process are derived from BALB/c mice.
Retired breeders may offer some advantages
for ascites production due to the
previously stretched abdominal musculature