It has been reported that the protein immobilization on plasmatreated PS surfaces could be improved in comparison to pristine PS
surfaces, as a consequence of the hydrophilic functionalization of
the oxygen-containing groups [20–22]. However, the corresponding protein binding depends on many factors in a complicated way
[1]. Therefore, over 100 types of fabrication and protein binding
parameters were evaluated by FN fluorescence immunoassay to
optimize these parameters.