Antioxidant activity was evaluated by the areas of negative peaks
at 517 nm and quantified by reference to a Trolox standard calibration
curve as an equivalent antioxidant concentration (μM). The
Trolox equivalent antioxidant capacity (TEAC) was defined as the
concentration of Trolox (mM) having the same activity as 1 mM of
the test compound. All measurements were performed in triplicate.
The theoretical Trolox equivalent concentration was calculated
according to Karaman, Tütem, Başkan, and Apak (2010) with slight
modifications. In this study, the theoretical Trolox equivalent concentration
was calculated by multiplying the content of the investigated
compound with its TEAC value. Theoretical Trolox equivalent concentration
(μM) = content (μM) × TEAC.