It was found that a dilution of 1:50 was the most efficient in
reducing the effects of PCR artefacts without losing alleles to overdilution (Fig. 1). It is recommended that several dilutions,
including the original PCR product, are analysed on the same
run to save time. There was a decrease of 29.4% in the average RFU
when anagen hair samples were diluted to a 1:50 concentration.