The biological stability test of cross-linked gelatin scaffolds using digestion with collagenases (type II, IV) and trypsin showed that scaffolds with higher glucose content (25% and 30%) were resistant to digestion with all three types of enzymes, as scaffolds maintained their pre-treatment shape. Scaffolds containing 20% glucose were partially degraded. Matrices containing 15% and less glucose were totally dissolved in collagenase and trypsin solution and no undigested scaffold fragments could be observed under optical microscope.