Preparation of liposomes
Amounts of 800 mg D5-18:3n-3 FAEE, 70 mg soy phosphatidylcholine,
10 mg Nile Red as a lipid soluble visual
tracer and 2 mg antioxidant solution [6% (w/v) butylated
hydroxy anisole, 6% (w/v) propyl gallate, 4% (w/v) citric
acid in propylene glycol] were dispensed into a 2 mL
screw cap bottle and dried under a stream of nitrogen.
Vials were then capped under argon and stored at
2208C until required. Liposomes were prepared by
adding 30 mL of diethyl ether and 300 mL of water and
whirly-mixing vigorously. The solution quickly went
cloudy and aggregates of liposomes ranged in size from
2–35 mm (determined by inverted microscopy)