Callus growth and somatic embryogenesis was recorded for the percentage of calli
forming somatic embryos, weight of calli and callus score. Calli were scored as: (1)
callus turned dark brown and no growth, (2) no differentiation of callus but remained
yellow, (3) callus-formed plantlets with 1–4 leaves and 1–8 roots, (4) callusformed
somatic embryos at proembryo, globular and heart shape stages and (5) callusformed
somatic embryos at proembryo, globular, heart, torpedo and mature embryo
stages.
2.5. Histology