No
matter the result of PCR or ELISA, HSYA has no effect on
Fas. It demonstrated that HSYA had little influence on the
death receptor pathway. All results indicate that the
mechanism of apoptosis by HSYA may conclude upregulating
the expression of Bax and Caspase-3, and
down-regulating that of Bcl-2 and mt P53. By means of a
series of effects, cascade reactions of caspases are triggered.
Then apoptosis signals enter the nucleus, further
perform apoptosis process and make cells occur apoptosis
finally (Fig. 8). However, there were still something that we
could not confirmed from these results. After we added
HSYA, Caspase-3 was activated. But we did ensure that its
activation was mediated by the mitochondrial pathway or
stimulated straightly by HSYA. The doubts like that still
need to be confirmed in the experiments in the future.