The study was conducted on male Wister rats (175-200gm) housed in polypropylene cages under standard conditions of temperature (22 ± 2°C), relative humidity (60 ± 5%) and light (12h light/dark cycle) were used. They were fed with standard diet and water. The food was withdrawn 18 hours before the experiment but allowed free access of water. All animal experiments were carried out in accordance with the guidelines of CPCSEA.