Alyxia reinwardtii Blume is a brush wood that widely found in Thailand, Indonesia, Malaysia, Philippines and
Vietnam. It is commonly known in Thai as “Cha-lood”. According to traditional Thai medicine, the flower
has been used for treating mental confusion and hallucination associated with high fever. Moreover, the
bark has been used for treatment of fainting, flatulence and fever. A. reinwardtii has been reported to
contain three classes of secondary metabolites, i.e., coumarin and its derivatives, trimeric-iridoid diglucoside
and iridolactone [1]. Coumarin has various pharmacological properties such as anti-nociceptive,
bronchodilator and anti-inflammatory properties [2]. Although, A. reinwardtii inner bark is widely used in
traditional Thai medicine but its pharmacognostic specification has not been established. Thin layer
chromatography (TLC) is the method conventionally used for qualitative investigation of the active
compounds in plant extracts because of the ease, simplicity and rapidity. TLC image analysis is a
computerised method that used to quantitate the pixel intensity of each compound band on TLC image,
transform to each chromatographic peak and calculate the peak area [3, 4]. ImageJ is a public domain Java
image processing open source software which can be used for TLC image analysis due to its easily
applicable [5]. This study aims to investigate the standardization parameters of A. reinwardtii inner bark in
Thailand with the special reference to coumarin marker.