In the last few years, the possibility of hydrolyzing starch at low temperatures for achieving energy savings has been investigated (Robertson et al., 2006). The product of this first step, called liquefaction, is a starch solution containing dextrines and small amounts of glucose. The liquefied starch is subject to saccharification at lower temperatures (60–70 °C) through gluco-amylase obtained generally from Aspergillus niger or Rhizopus species ( Pandey et al., 2000 and Shigechi et al., 2004). This study was therefore aimed at investigating the ethanol producing abilities of two local varieties of cassava using indigenous yeast species from palm wine compared with commercial bakers’ yeast after exogenous enzyme hydrolysis.