In addition, by searching the Genomics Date Base(DB),ADP-glucose pyrophosphorylase(shrunkengene, SH51,AP004317), a gene involved in starch biosynthesis and located on chromosome 1, was also chosen for marker development.
By comparing the sequence of each gene (Table 2) among japonica varieties, we developed a total of nine DNA markers(Table 3).
Examples of PCR amplicons of different marker types amongjaponica rice varieties are shown in Figure 1.