The 95 strains of yeast isolated from commercial blueveined
cheese are listed in Table 1. These strains were
identified by nucleotide sequence analysis of the specific
amplification of the intertranscribed spacer region
(White et al., 1990). Four yeast strains isolated from
kefir were also investigated; they have been identified
as Saccharomyces cerevisiae K1, K. lodderae K2, K.
marxianus K3, and Candida humilis K4. Organisms
were cultured in yeast-peptone-dextrose (YPD) medium
containing 1% yeast extract (Difco, Detroit, MI),
2% polypeptone (Nihon Seiyaku, Tokyo, Japan), and
2% glucose, at 27°C for 48 h