The suitability of any protease for inclusion in detergent formulation
is dependent on its stability and compatibility with
detergent components [29,30]. Besides, the enzyme should be
alkaline in nature and thermostable [30]. However, the stability
and compatibility of any component should not be the only
pre-requisite for its inclusion in detergent formulation. To save
energy from heating the water to be used for washing, the ability
of the detergent components to perform wash function at
lower (room) temperature should also be addressed [3]. There
are only few reports published showing the activity of protease at
room temperature [30,31]. The protease secreted by B. subtilis
strain in the present study remained active at room temperature
and at alkaline pH rage favoring its inclusion in laundry
detergents