The chromatogram was developed at a flow rate of 1.0 ml/min by eluting the sample with mobile phase A (0.1 M ammonium acetate, pH 6.5) and mobile phase B (0.1 M ammonium acetate, acetonitrile, methanol, 44:46:10, v/v/v, pH 6.5) as described by Caceres et al. (2014). Samples were independently analysed in triplicate and results are expressed as mg
GABA/100 g.