Lift loopful many times from the suspension surface, observe whether the bacteria form a sticky suspension lifted like a
thread with a needle ose. When the suspension turned been like a slimy, sticky and lifted like a thread with a needle
ose, meaning Gram negative (-). Conversely, if the suspension remains dilute, not rose like the needle thread loop,
meaning Gram positive (+). The selected bacterium is gram-negative bacteria. To prove and validate whether these
bacteria are gram-negative bacteria producing exopolysaccharide subsequently grown on the Mac Concey medium
(Oxoid CM 0007) is characterized by the presence of slime formed.