Effective vector control as an integrative component of malaria control relies heavily on our understanding of the community structure, seasonal abundance, and infection status of the vector species. Anthropogenic land use activities and insecticide-based control measures have resulted in major shifts of mosquito species abundance and changes in biting behavior, which require continued monitoring of vectors [1]. In this study, we used both CDC light traps (indoor and outdoor) and the cattle bait method to assess the anopheline mosquito abundance in western Thailand in light of the malaria elimination campaign being carried out in this region. This study further revealed the diverse anopheline fauna in this region, confirmed the major vectorial status of several mosquito species, and identified new potential vectors.