By optimizing fermentation (Huijbert et al.
1996) could increase the PHA content to 88% of cell dry
weight and PHA productivity to 4.94 g/L/h. Significant
improvement on biomass and polymer production has been
observed in our experiment, raising CDW yield to 6.75 g/L
and maximizing the PHA accumulation (3.05 g/L) after 48 h.
By using a fed-batch culture with P. putida strain using oleic
acid as carbon source (Lee et al. 1999) were able to enhance
cell concentration, PHA concentration and PHA content
obtained in 34 h, resulting in the PHA productivity of 1.79
g/L/h.