Cordycepin is one of the most versatile metabolites of Cordyceps militaris. When C. militaris G81-3, the
mutant obtained by a proton beam irradiation, was cultivated by liquid surface culture, cordycepin was
found to crystallize in the medium due to high cordycepin concentration. Because the cordycepin crystals
strongly attached to the mycelial mat, complete recovery of cordycepin was difficult. To prevent cordycepin
crystallization, increase of the initial medium volume was examined to decrease the condensation
rate by vaporization, in combination with decrease of the initial substrate level. Besides, addition of the
water to the culture bottle was examined to cancel out the medium condensation. A 7.4 g/L of cordycepin
was obtained without crystallization by the former method. The upper limit of cordycepin production,
14.3 g/L, was obtained by the latter method and this value was the highestlevel of allthe previous reports.
A 2.5-fold scale-up of the culture slightly declined the production rate, however, the amount of cordycepin
production was properly 2.5 times higher than that of the small scale. In addition, differences in
sensitivities to the substrates between the mutant and the wild-type strain are discussed
Cordycepin is one of the most versatile metabolites of Cordyceps militaris. When C. militaris G81-3, themutant obtained by a proton beam irradiation, was cultivated by liquid surface culture, cordycepin wasfound to crystallize in the medium due to high cordycepin concentration. Because the cordycepin crystalsstrongly attached to the mycelial mat, complete recovery of cordycepin was difficult. To prevent cordycepincrystallization, increase of the initial medium volume was examined to decrease the condensationrate by vaporization, in combination with decrease of the initial substrate level. Besides, addition of thewater to the culture bottle was examined to cancel out the medium condensation. A 7.4 g/L of cordycepinwas obtained without crystallization by the former method. The upper limit of cordycepin production,14.3 g/L, was obtained by the latter method and this value was the highestlevel of allthe previous reports.A 2.5-fold scale-up of the culture slightly declined the production rate, however, the amount of cordycepinproduction was properly 2.5 times higher than that of the small scale. In addition, differences insensitivities to the substrates between the mutant and the wild-type strain are discussed
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