Screening of antibiotic-producing strains
Primary screening test
The anti microbial activities of the isolates were tested by cross-streak plate method employing nutrient agar medium for bacteria and potato dextrose agar medium for fungi. The media were sterilized by autoclaving at 121°C. For 15 min and the molten sterile media were cooled to 40 to 45°C poured in to Petri plates (4 inch diameter) and allowed to solidify. Each plate was streaked with one isolate at the center and incubated at 20°C for 7 days. After 7 days, test organisms were streaked perpendicular to the growth of the isolate; 24 h cultures of bacteria, and 4 day cultures of fungi.