In order to check whether or not pI-control with these dyes
had any effect on the reproducibility of protein spot assignment in 2D-PAGE, we usedE. colilysate as the analyte proteome
in the same manner as described before (equipment from GE
Healthcare, Freiburg, Germany; HPE FlatTop Tower from Serva,
Heidelberg, Germany[4]). Briefly, the analyte proteome was