No spermatozoa were observed in the ampulla in either treatment group. There were no differences in the number of spermatozoa between treatments in the isthmus. In the UTJ section, the number of spermatozoa in the flushing fluid (mMS group vs. PFM group = 8.8 ± 2.6 vs. 7.1 ± 1.7 × 103 spermatozoa/suspension) and in the crypts (mMS group vs. PFM group = 178.8 ± 74.4 vs. 229.6 ± 125.1 spermatozoa/mm) did not differ between the groups.
The mean number of PMNs in the PFM group was significantly lower(P < 0.05) than in the mMS group in both the uterine body and horns (Fig. 3).
The numbers of spermatozoa phagocytosed by PMNs in the uterus did not differ between the groups. However, the number of non-phagocytosed sperm(i.e. outside PMNs) in the uterus horn was significantly higher in PFM than in mMS groups.