Estimation of Cellulase enzyme activity
1 ml 0.05 M Na-citrate(pH 4.8) is added to a test tube of volume at least 25 ml. 0.5 ml enzyme is diluted in citrate buffer with Whatman No. 1 filter paper strip(l.0 6.0 cm or 50 mg).
Two dilutions are made of each enzyme sample for investigation. One dilution should release slightly more and one slightly less than 2.0 mg(absolute amount) of glucose reducing sugars as glucose) in the reaction conditions.
investigation