3 ml sample volume was finally chosen, as (fortified) milk contained very less concentration of vitamin D2 (15 ng/ml) and there was difficulty in quantification of low sample volume due to interfering components present in milk.
3 ml sample volumewas finally chosen, as (fortified) milk contained very less concentrationof vitamin D2 (15 ng/ml) and there was difficulty in quantificationof low sample volume due to interfering componentspresent in milk.