During the second PCR round, the obtained
fragments were reampli¢ed by using the Bacterial
primers P338F and P518r in one and the same PCR protocol
(Table 2). Since all mentioned groups in Table 1
belong to the domain of the Bacteria, the Bacterial primers
P338F and P518r used in the second PCR round should
reamplify all fragments obtained after the ¢rst PCR
round. After PCR, samples were stored at 4³C (few hours)
or at 320³C (days)