Bacterial Count Measurements
For the determination of bacterial counts, 1 g of chicken
breast sample was weighed in a sterile bag (NascoWhirl-Pak, Janesville, WI) and homogenized with 9 mL of
0.1% sterilized peptone water in a stomacher (Lab blender 400, Seward Laboratory, Worthing, U.K.) for 2 min. Serial
dilutions were prepared using 0.1% sterilized peptone water. For the determination of ST and total aerobic bacterial
counts, 1 mL of the serial dilution was placed in LB broth (Difco) and plate count agar (PCA; Difco). The
plates were incubated aerobically at 37C for 24–48 h. For the determination of total anaerobic bacterial counts, 1 mL
of the serial dilution was placed in the PCA (Difco) and incubated anaerobically at 37C for 24–48 h. Microbial
population was counted using a colony counter (C-C03, Chang Shin Scientific, Busan, Korea) and expressed as log
cfu/g sample.
Bacterial Count MeasurementsFor the determination of bacterial counts, 1 g of chickenbreast sample was weighed in a sterile bag (NascoWhirl-Pak, Janesville, WI) and homogenized with 9 mL of0.1% sterilized peptone water in a stomacher (Lab blender 400, Seward Laboratory, Worthing, U.K.) for 2 min. Serialdilutions were prepared using 0.1% sterilized peptone water. For the determination of ST and total aerobic bacterialcounts, 1 mL of the serial dilution was placed in LB broth (Difco) and plate count agar (PCA; Difco). Theplates were incubated aerobically at 37C for 24–48 h. For the determination of total anaerobic bacterial counts, 1 mLof the serial dilution was placed in the PCA (Difco) and incubated anaerobically at 37C for 24–48 h. Microbialpopulation was counted using a colony counter (C-C03, Chang Shin Scientific, Busan, Korea) and expressed as logcfu/g sample.
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