Differentiated tissue in Panax ginseng cultures was found to be very efficacious for saponin production.
In order to increase the yield of saponins and preserve culture stability we were testing different
plant growth regulators (PGR) and auxin/cytokinin combinations to regulate a level of tissue differentiation.
For this purpose we used transverse thin cell layers (tTCLs) of adventitious roots of Panax ginseng.
Adventitious roots were cultivated in Shenk and Hildebrand (SH) liquid medium supplemented with IBA
(24.6 M). Callus formation and root multiplication of adventitious root tTCLs was evaluated after 4 and
following 12 weeks of cultivation, respectively, on SH basal medium containing various auxins (3 mg l
−1)
or cytokinins (0.2 or 0.02 mg l
−1) or their combinations. We found that kinetin (Kin) in combination with
auxin benzo[b]selenienyl acetic acid (BSAA), naphthalene acetic acid or indole-3-butric acidis the best
for biomass production and following root multiplication. These combinations were tested in previously
selectedmost suitable large-scale system—a temporary immersionsystemRITA. The best saponinproduction
(15.94 ± 1.89 mg g−1 dry weight) and growth value (5.62 ± 0.34) was reached on medium containing
BSAA and Kin combination.