The coding region of the wild-type N-Rne or the hyperactive N-Rne mutant under the control of the T7 promoter were integrated into the endA gene in the chromosome of strain BL21(DE3) (Fig. 1 A).
The coding region of the wild-type N-Rne or the hyperactive N-Rne mutant under the control of the T7 promoter were integrated into the endA gene in the chromosome of strain BL21(DE3) (Fig. 1 A).