carrots (Daucus carota) are a common source of β-carotene and they are often consumed as processed products (e.g., soups, juices, purees, etc.). One major purpose of food processing is food preservation. The shelf-life of food products may be enhanced by destruction of pathogenic microorganisms and inactivation of spoilage microorganisms and enzymes (Ramaswamy and Marcotte, 2006). Processing might also affect β-carotene in different ways. In this chapter, the effect of processing of carrot-based products on β-carotene stability on the one hand, and on β-carotene bioaccessibility on the other hand, is discussed.