Control of anthracnose on chili fruit The chili fruits (Capsicum anuum L. var. annuum) collected from chili field at Agriculture field, Khorkhori, Rajshahi district were disinfested with 0.5 % sodium hypochlorite before making a tiny wound on each chili fruit with a sterilized needle.
11 Rahman et al.
Then, the wounded chili fruit was immersed in 2000 mg/l, 30 days old of each antifungal metabolite for an hour at room temperature. Then, 0.5 ml of spore suspension of C. capsici (10x105spores/ml) was dropped on the wounded area on chili fruit. The inoculated chili fruits were kept in plastic box and incubated for 4 days in plant growth chamber (25oC, 12 h of light and 80 % of water capacity content). The percentage of disease inhibition was then observed, recorded and compared with the control treatment (wounded chili fruits soaking in 2% methanol). Each treatment comprised of four replicates with ten fruits in each replicate. The percentage of disease inhibition was calculated by using the formula; ((RC-RT)/RC) ×100, when RC was the mean of diseased lesion radius on chili in the control and RT was the mean of diseased lesion radius on chili in the treatment with antifungal metabolite.