Effect of temperature . Uptake experiments were performed at controlled pH and at room temperature by shaking 0.05 g of dry chitosan in a series of flasks containing with 10 mL metal ion solutions at pH 3.
The flasks were agitated on a shaker at 150 rpm for 4 h while keeping the temperature at 20 , 25 , 30 and 40 C. After adsorption, solution was filtered and the residual concentration of the metal ions was determined.