Flow cytometry was performed to evaluate cell viability and types of death, BAX
and BCL-2 expression, alterations on cell cycle and mitochondrial membrane
potential (MP) and the redox intracellular environment through determination of
ROS production (superoxide radical and peroxides) and expression of reduced
glutathione (GSH). Comet assay was used in order to assess DNA damage
induced by different concentrations of AA.