Liquid Chromatography
A Shimadzu Prominence LC system
containing a CBM-20A system controller,
two LC-20AD pumps, a semi-micro
gradient mixer SUS-20A, and a SIL-20AC
autosampler was used. Separation
was performed on a GL Science Inertsil
HILIC 5μm (150x3mm) column at a temperature of 40°C with a mobile
phase of (A) acetonitrile + 10mM
ammonium acetate and (B) water +
10mM ammonium acetate at a flow of
0.5mL/min. The gradient using normal
phase conditions is listed in Table 1.
An injection volume of 5μL was used.