The economically significant production of carbon-neutral biodiesel from microalgae has been hailed as
the ultimate alternative to depleting resources of petro-diesel due to its high cellular concentration of lipids,
resources and economic sustainability and overall potential advantages over other sources of biofuels.
Pertinent questions however need to be answered on the commercial viability of large scale
production of biodiesel from microalgae. Vital steps need to be critically analysed at each stage. Isolation
of microalgae should be based on the question of whether marine or freshwater microalgae, cultures
from collections or indigenous wild types are best suited for large scale production. Furthermore, the
determination of initial sampling points play a pivotal role in the determination of strain selection as well
as strain viability. The screening process should identify, purify and select lipid producing strains. Are
natural strains or stressed strains higher in lipid productivity? The synergistic interactions that occur naturally
between algae and other microorganisms cannot be ignored. A lot of literature is available on the
downstream processing of microalgae but a few reports are available on the upstream processing of microalgae
for biomass and lipid production for biodiesel production. We present in this review an empirical
and critical analysis on the potential of translating research findings from laboratory scale trials to full
scale application. The move from laboratory to large scale microalgal cultivation requires careful planning.
It is imperative to do extensive pre-pilot demonstration trials and formulate a suitable trajectory
for possible data extrapolation for large scale experimental designs. The pros and cons of the two widely
used methods for growing microalgae by photobioreactors or open raceway ponds are discussed in detail.
In addition, current methods for biomass harvesting and lipid extraction are critically evaluated. This
would be novel approach to economical biodiesel production from microalgae in the near future. Globally,
microalgae are largest biomass producers having higher neutral lipid content outcompeting terrestrial
plants for biofuel production. However, the viscosities of microalgal oils are usually higher than that
of petroleum diesel.
The economically significant production of carbon-neutral biodiesel from microalgae has been hailed asthe ultimate alternative to depleting resources of petro-diesel due to its high cellular concentration of lipids,resources and economic sustainability and overall potential advantages over other sources of biofuels.Pertinent questions however need to be answered on the commercial viability of large scaleproduction of biodiesel from microalgae. Vital steps need to be critically analysed at each stage. Isolationof microalgae should be based on the question of whether marine or freshwater microalgae, culturesfrom collections or indigenous wild types are best suited for large scale production. Furthermore, thedetermination of initial sampling points play a pivotal role in the determination of strain selection as wellas strain viability. The screening process should identify, purify and select lipid producing strains. Arenatural strains or stressed strains higher in lipid productivity? The synergistic interactions that occur naturallybetween algae and other microorganisms cannot be ignored. A lot of literature is available on thedownstream processing of microalgae but a few reports are available on the upstream processing of microalgaefor biomass and lipid production for biodiesel production. We present in this review an empiricaland critical analysis on the potential of translating research findings from laboratory scale trials to fullscale application. The move from laboratory to large scale microalgal cultivation requires careful planning.It is imperative to do extensive pre-pilot demonstration trials and formulate a suitable trajectoryfor possible data extrapolation for large scale experimental designs. The pros and cons of the two widelyused methods for growing microalgae by photobioreactors or open raceway ponds are discussed in detail.In addition, current methods for biomass harvesting and lipid extraction are critically evaluated. Thiswould be novel approach to economical biodiesel production from microalgae in the near future. Globally,microalgae are largest biomass producers having higher neutral lipid content outcompeting terrestrialplants for biofuel production. However, the viscosities of microalgal oils are usually higher than thatof petroleum diesel.
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