Western blots were used to investigate the effect of antisense repression of UGD expression
on the level of UGD protein in the leaf roll of transgenic sugarcane. Western blot analysis
was carried out using a polyclonal antiserum raised against UGD from sugarcane. In
agreement with the reduction shown in the transcript levels of UGD in pAUGdf510 transgenic
lines, UGD protein amount was reduced in selected lines as compared to control plants.
Among the transgenic lines, A-34 had the lowest level of UGD protein, followed by A-32.