2.2. Sampling
Depending on size, between 20 and 150 larvae were collected
using a 200 μm dip net, 1 h after the first daily feeding. Samples were
collected daily starting at 0 dph (free embryos) through to 6 dph,
every 2 days from 8 dph until 20 dph, and every 4 days thereafter
until 40 dph. After sampling, larvae were anesthetized with tricaine
methanesulfonate (MS 222), rinsed with distilled water, freeze-dried,
and stored at −80 °C until analysis.