The process of fermentation is significantly influenced by various physical and chemical factors. In addition, phytase production is affected by growth conditions, the strain and substrate used for culture, and the availability of nutrients [2]. Statistical plans are currently used to find ways to enhance phytase production at a reduced cost. The use of response surface methodology (RSM) in biotechnological processes has gained great importance in the optimization of enzyme production because it helps to determine the optimum parameters for enzyme activity