A total of 120 beef patties were formed, and placed on styrofoam trays.
The tray with the patty was introduced in a pouch made of a polyethylene and polyamide laminate of water vapour permeability 5–7 g/m2/24 h at 23°C and oxygen permeability 40–50 ml/m2/24 h at 23°C.
The pouch was filled with a gas mixture of 70% O2+20% CO2+10% N2, and sealed.
The patties were stored for 20 days at 2+1°C in the dark.
Three packs were opened for subsequent analysis of each formulation every 4 days of storage. The entire experiment was replicated twice.