Reactivity of rabbit anti-LipL32 against
LipL32
LipL32 was purified as described in Materials
and Methods. Purified LipL32 protein was subjected
to 10% SDS-PAGE and gel was stained
with Coomassie brilliant blue (Fig. 2A). LipL32
was used to immunize a rabbit. Reactivity of
rabbit anti-LipL32 against purified LipL32
was demonstrated by Western blot as shown
in Figure 2B. Purified LipL32 was probed with
pre-immunized rabbit serum (Fig. 2B, Lane 1),
serum from a rabbit immunized with LipL32
(Lane 2) and rabbit IgG purified from a LipL32-
immunized rabbit (Lane 3). Serum or purified