and centrifuged at 12,000 g for 15 minutes. Fifty units
of Benzonase (250 units/mL; Sigma) was added to the
mixture and suitably stored at 80 C until use after
quantitation by the Bradford method. For 2-DE analysis,
pH 3e10 immobilized pH gradient (IPG) strips
(Amersham Biosciences, UK, Ltd) were rehydrated in
swelling buffer containing 7 M urea, 2 M thiourea, 0.4%
(w/v) Dithiothreitol, and 4% (w/v) CHAPS.