INDUCTION OF MULTIPLE MUTATION UNE IN WHEAT BY THE USE OF N-ETHYL-N-NITROSO UREA
The mutant line carrying many mutated characters was induced after mutagenic treatment of seeds of spring wheat variety 'Rena' with N-ethyl-N-nitroso urea (2, 4, 6 mM). This mutant line was named 'KS-5'. The most characteristic change in this mutant line is the decrease in the number of days to maturity when compared with 'Rena'. The mutant line is ready for harvest at the same time as variety 'Kosutkz', which is the earliest, of all the former Czechoslovak varieties. The mutant line 'KS-5' is shorter by 10 cm (average length of the stem is about 65 cm) than the parent variety 'Rena' and also more resistent to wheat powdery mildew. The mutant was selected from a population sown in high density. This was achieved by sowing compact ears at a distance of 15 x 15 cm. The mutant line was sown in autumn. After multiple selection for improved overwintering, it was estimated that the critical temperature CT^, for this mutant line was -14.5°C. In following generations other mutated characters were expressed. It was found that this mutant line reacts positively to the high plant density obtained with higher rate of sown seeds. An optimal plant density was obtained for this mutant line with about 800 seeds/m2 (Table 1).
INDUCTION OF MULTIPLE MUTATION UNE IN WHEAT BY THE USE OF N-ETHYL-N-NITROSO UREAThe mutant line carrying many mutated characters was induced after mutagenic treatment of seeds of spring wheat variety 'Rena' with N-ethyl-N-nitroso urea (2, 4, 6 mM). This mutant line was named 'KS-5'. The most characteristic change in this mutant line is the decrease in the number of days to maturity when compared with 'Rena'. The mutant line is ready for harvest at the same time as variety 'Kosutkz', which is the earliest, of all the former Czechoslovak varieties. The mutant line 'KS-5' is shorter by 10 cm (average length of the stem is about 65 cm) than the parent variety 'Rena' and also more resistent to wheat powdery mildew. The mutant was selected from a population sown in high density. This was achieved by sowing compact ears at a distance of 15 x 15 cm. The mutant line was sown in autumn. After multiple selection for improved overwintering, it was estimated that the critical temperature CT^, for this mutant line was -14.5°C. In following generations other mutated characters were expressed. It was found that this mutant line reacts positively to the high plant density obtained with higher rate of sown seeds. An optimal plant density was obtained for this mutant line with about 800 seeds/m2 (Table 1).
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