2.3. SFJ heating
Heat treatment of the different SFJ systems was performed by
placing the sample solutions in a water bath (PolyScience, Illinois,
USA) that was set to heating temperature that would allow the SFJ
to reach and maintain the temperature pre-determined in the
CCRD. To ensure that the assigned temperature is reached, a thermometer
was placed in the cold point of the SFJ. Upon reaching the designated temperature, appropriate volumes of stock AA (Ajax
Finechem, New South Wales, Australia) solution (100,000 mg/L)
were transferred into the heated SFJs to introduce initial AA concentrations
according to the values determined in the CCRD combinations.
The AA-supplemented SFJs were then immediately
gently agitated to homogenize the vitamin distribution throughout
the heating medium. The AA solutions were then subjected to the
pre-determined heating temperature for the pre-determined
length of time, after which the solutions were immediately
cooled in an ice water bath.
2.3. SFJ heatingHeat treatment of the different SFJ systems was performed byplacing the sample solutions in a water bath (PolyScience, Illinois,USA) that was set to heating temperature that would allow the SFJto reach and maintain the temperature pre-determined in theCCRD. To ensure that the assigned temperature is reached, a thermometerwas placed in the cold point of the SFJ. Upon reaching the designated temperature, appropriate volumes of stock AA (AjaxFinechem, New South Wales, Australia) solution (100,000 mg/L)were transferred into the heated SFJs to introduce initial AA concentrationsaccording to the values determined in the CCRD combinations.The AA-supplemented SFJs were then immediatelygently agitated to homogenize the vitamin distribution throughoutthe heating medium. The AA solutions were then subjected to thepre-determined heating temperature for the pre-determinedlength of time, after which the solutions were immediatelycooled in an ice water bath.
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