Nitric oxide (NO) is a highly reactive signaling molecule and plays a variety of physiological roles in
plants. The research on the application of NO to postharvest preservation of flowers and fruits shows
great promise in recent years. However, the physiological mechanism of exogenous NO to affect cut
flowers is not very clear. Therefore, an experiment was conducted to study the effect of exogenous NO
on the vase life and physiological basis of Dianthus caryophyllus L. variety ‘Monte’. In the present study,
0.1mmolL−1 sodium nitroprussiate (SNP) was used as the NO donor, and 5mol L−1 methylene blue
(MB-1) was used as its scavenger based on the preliminary experiment. We evaluated the physiological
index including increase of stem weight, malondialdehyde (MDA) concentration and lipoxygenase (LOX)
activity, and cell protection enzymes activities of superoxide dismutase (SOD), peroxidase (POD), catalase
(CAT) and ascorbate peroxidase (APX). The results showed that exogenous NO could significantly extend
the vase life of cut carnation flowers and markedly increase fresh mass. The balance of water metabolism
and the activities of SOD, POD, CAT and APX also showed improvement, while the production of MDA
content and LOX activity were obviously decreased. The results suggest that exogenous NO could delay
petal wilting in carination cut flowers, maintain water metabolism, the antioxidative enzymes activity
and mass-eliminate reactive oxygen species (ROS) and as well as cell membrane stability. Moreover, the
results indicated that MB-1 had the ability to reverse the active effects of NO on different physiological
indexes. Therefore, the vase life of cut carnation flowers was markedly extended by SNP treatment.
Nitric oxide (NO) is a highly reactive signaling molecule and plays a variety of physiological roles inplants. The research on the application of NO to postharvest preservation of flowers and fruits showsgreat promise in recent years. However, the physiological mechanism of exogenous NO to affect cutflowers is not very clear. Therefore, an experiment was conducted to study the effect of exogenous NOon the vase life and physiological basis of Dianthus caryophyllus L. variety ‘Monte’. In the present study,0.1mmolL−1 sodium nitroprussiate (SNP) was used as the NO donor, and 5mol L−1 methylene blue(MB-1) was used as its scavenger based on the preliminary experiment. We evaluated the physiologicalindex including increase of stem weight, malondialdehyde (MDA) concentration and lipoxygenase (LOX)activity, and cell protection enzymes activities of superoxide dismutase (SOD), peroxidase (POD), catalase(CAT) and ascorbate peroxidase (APX). The results showed that exogenous NO could significantly extendthe vase life of cut carnation flowers and markedly increase fresh mass. The balance of water metabolismand the activities of SOD, POD, CAT and APX also showed improvement, while the production of MDAcontent and LOX activity were obviously decreased. The results suggest that exogenous NO could delaypetal wilting in carination cut flowers, maintain water metabolism, the antioxidative enzymes activityและมวลกำจัดชนิดปฏิกิริยาออกซิเจน (ROS) และเช่นเป็นเซลล์เมมเบรนความมั่นคง นอกจากนี้ การผลระบุว่า MB 1 มีความสามารถในการกลับรายการผลจากการใช้งานไม่แตกต่างกันในสรีรวิทยาดัชนี ดังนั้น อายุการปักแจกันของคาร์เนชั่นดอกไม้ตัดได้อย่างเด่นชัดแบบขยาย โดย SNP รักษา
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