the fermentation medium contained 100 g of wheat bran grain (size 5), humidified with a solution containing nitrogen source (ammonium sulfate) and inducer (sunflower soapstock)in a cocentration of 3% (w/w).
Concentration of ammonium sulfate used is related to the concentration of nitrogen required.
For that reason, this nutrient was dissolved in the amount of water as established in the experimental design.
Nitrogen concentration and the volume of liquid (moisture-related) are indicated in the experimental design (Table 1). 
The fermentation medium was autoclaved at 121 0C at 1 atm for 15 min