In present experiment the B. subtilis LS 1-2 obtained after fermentation was mixed with carrier (corn–soybean meal) in such a way that addition of 1.5, 3.0 and 4.5 g/kg of diet, would give 107,
108 and 109 cfu/kg diet. Efficacy for most probiotics in animals could be demonstrated with the daily intake of 107–109 microorganisms