Bacterial Stalk rot of maize is one of the most destructive diseases in several maize growing regions of Thailand. The use of the host resistance would be a more desirable method for disease control. This test was conducted to screen sweet corn accessions for resistance to bacterial stalk rot using toothpick inoculation technique. Fourteen day-old seedlings of thirty sweet corn accessions were artificially inoculated and screened against four virulence strains of bacterial stalk rot under greenhouse condition. Percent of disease incidence and disease index and were recorded after 6 days of inoculation. Only two out of thirty corn accessions were considered resistant to all tested bacterial strains. These accessions were SM2429 and SM1625 and may be useful as resistant source in corn breeding program. Furthermore, we found that use of toothpick inoculation technique made it possible to screen large number of corn accessions in a short period of time.